EnPlusOne Biosciences

EnPlusOne Biosciences is a biotechnology company based in Watertown, MA, founded in 2022. The company specializes in an enzymatic RNA synthesis platform known as ezRNA™, which facilitates the scalable manufacturing of RNA therapeutics and vaccines. This innovative platform utilizes water-based, template-free reactions, allowing for efficient de novo RNA synthesis and the incorporation of various natural and modified nucleotides.

EnPlusOne aims to address the increasing demand for RNA therapeutics by providing a greener alternative to traditional chemical methods. Their approach promises sustainable production with reduced capital expenditures and less hazardous waste. The company has successfully raised $12 million in seed funding and an additional $10 million to enhance its partnerships and synthesis capabilities. With a small team, EnPlusOne is focused on advancing its platform to meet the evolving needs of RNA developers.